4RDH
Crystal structure of E. coli tRNA N6-threonylcarbamoyladenosine dehydratase, TcdA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 270r |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.686, 97.190, 84.478 |
| Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.15088 |
| Rwork | 0.148 |
| R-free | 0.20396 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.092 | 0.375 |
| Number of reflections | 57374 | |
| <I/σ(I)> | 24 | 5.38 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 25% (w/v) PEG 3350, 0.1 M Hepes, pH 7.5 and 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
