4RDB
Crystal structure of an immunoreactive 32 kDa antigen PG49 (PG_0181) from Porphyromonas gingivalis W83 at 1.45 A resolution (PSI Community Target, Nakayama)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184,0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.636, 86.821, 90.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.411 - 1.450 |
R-factor | 0.1191 |
Rwork | 0.117 |
R-free | 0.15690 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.605 |
Data reduction software | XDS |
Data scaling software | XSCALE (January 10, 2014 BUILT=20140307) |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.411 | 1.500 | |
High resolution limit [Å] | 1.450 | 3.120 | 1.450 |
Rmerge | 0.083 | 0.027 | 0.714 |
Number of reflections | 55908 | 5928 | 5264 |
<I/σ(I)> | 13.38 | 39.2 | 2.3 |
Completeness [%] | 99.1 | 98.7 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.17M sodium acetate, 15.0% Glycerol, 28.5% polyethylene glycol 4000, 4.0% Acetone, 0.1M TRIS pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |