4RD4
Structure of aIF2 gamma from sulfolobus solfataricus bound to gdpnp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.390, 60.950, 143.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.441 - 1.300 |
| R-factor | 0.1684 |
| Rwork | 0.167 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aho |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 1.290 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.055 | 0.933 |
| Number of reflections | 120182 | |
| <I/σ(I)> | 10.32 | 0.92 |
| Completeness [%] | 97.2 | 89.9 |
| Redundancy | 3.45 | 3.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 60%MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
