4RCK
Crystal Structure of Uncharacterized Membrane Spanning Protein from Vibrio fischeri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 65 |
| Unit cell lengths | 49.734, 49.734, 343.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.508 - 2.999 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.845 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1745)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 9679 | |
| <I/σ(I)> | 9.8 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.5 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Magnesium formate, 20 %(w/v) PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
