4RCI
Crystal structure of YTHDF1 YTH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979237 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.519, 82.136, 123.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.970 |
| R-factor | 0.1903 |
| Rwork | 0.189 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3i |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.17) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.660 | 48.660 | 2.020 |
| High resolution limit [Å] | 1.970 | 9.030 | 1.970 |
| Rmerge | 0.088 | 0.025 | 0.967 |
| Total number of observations | 4209 | 29482 | |
| Number of reflections | 57668 | ||
| <I/σ(I)> | 17.2 | 57 | 2.3 |
| Completeness [%] | 99.9 | 98.5 | 99.7 |
| Redundancy | 7.3 | 6.1 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 25% PEG-3350, 0.2 M potassium thiocyanate, vapor diffusion, temperature 293K |
