4RCI

Crystal structure of YTHDF1 YTH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979237
Spacegroup name	P 21 21 21
Unit cell lengths	79.519, 82.136, 123.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.970
R-factor	0.1903
Rwork	0.189
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3i
RMSD bond length	0.013
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	Aimless (0.2.17)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.660	48.660	2.020
High resolution limit [Å]	1.970	9.030	1.970
Rmerge	0.088	0.025	0.967
Total number of observations		4209	29482
Number of reflections	57668
<I/σ(I)>	17.2	57	2.3
Completeness [%]	99.9	98.5	99.7
Redundancy	7.3	6.1	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	25% PEG-3350, 0.2 M potassium thiocyanate, vapor diffusion, temperature 293K