4RBQ

32 base pair oligo(U) RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	H 3 2
Unit cell lengths	42.890, 42.890, 266.936
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.790 - 1.050
R-factor	0.1312
Rwork	0.131
R-free	0.15370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IDEAL A-FORM RNA
RMSD bond length	0.004
RMSD bond angle	0.898
Data reduction software	MOSFLM
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.3_927))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.800
High resolution limit [Å]	1.050	1.050
Rmerge	0.057
Number of reflections	45101
<I/σ(I)>	20.1
Completeness [%]	100.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	50 mM sodium cacodylate, pH 6.5, 200 mM potassium chloride, 75 mM magnesium acetate, 900 mM lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K