4RA2
PP2Ca
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 77 |
Detector technology | DIFFRACTOMETER |
Collection date | 2014-03-30 |
Detector | SYNTEX |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.714, 90.714, 105.443 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.810 - 1.940 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a6q |
RMSD bond length | 0.007 |
RMSD bond angle | 1.091 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.940 | 1.950 |
Number of reflections | 35772 | |
Completeness [%] | 95.4 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 277.15 | 15(v/v)% PEG8000, pH 5.5, 50mM K2HPO4, 15% 2mMDTT, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |