4R9V
Crystal structure of sialyltransferase from photobacterium damselae, residues 113-497 corresponding to the gt-b domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97607 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.444, 56.523, 87.375 |
| Unit cell angles | 90.00, 119.95, 90.00 |
Refinement procedure
| Resolution | 41.350 - 2.300 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z4t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.075 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.067 | 0.320 |
| Number of reflections | 17873 | |
| <I/σ(I)> | 16.9 | 3.6 |
| Completeness [%] | 99.1 | 99.2 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 294 | 20% (w/v) PEG-1000, 100mM imidazole, pH 8.0, 200mM Calcium Acetate, VAPOR DIFFUSION, temperature 294K |
