4R8U
S-SAD structure of DINB-DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.950, 57.210, 110.600 |
| Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
| Resolution | 48.230 - 2.300 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.21950 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.361 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.329 | 2.326 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 93883 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 18% MPD, 0.1M Citrate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298KK |
