4R8G

Crystal Structure of Myosin-1c tail in complex with Calmodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	105.447, 105.447, 288.068
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.590 - 3.503
R-factor	0.2455
Rwork	0.242
R-free	0.30440
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.386
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Number of reflections	21359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289.3	2.0M ammonium sulfate, 5% 1,4-dioxane, MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289.3K