4R86
Crystal Structure of Aminoglycoside/Multidrug Efflux System AcrD from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 91.167, 91.167, 147.402 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.584 - 3.001 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.784 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1745)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 14557 | |
| <I/σ(I)> | 10.6 | 2.4 |
| Completeness [%] | 98.9 | 99.8 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 47.1 % w/v PEG 1000, 150 mM Tris pH 8.0, 30 mM Potassium Bromide, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
