4R7Y
Crystal structure of an active MCM hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-05 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 118.902, 118.902, 199.317 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.830 - 2.700 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SsoMCM N-terminal domain (2VL6) PfMCM ATPase domain (related entry 4R7Z) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 43329 | |
| <I/σ(I)> | 14.8 | 1.79 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 6.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 281 | 10.8 mg/mL Sso-PfMCM; 5 mM ADP mixed 1:1 with 100 mM HEPES, pH 7.6; 350 mM MgCl2; 3 % (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
