4R7N
Fab C2E3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-13 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 178.100, 208.410, 214.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.775 - 3.450 |
| R-factor | 0.2379 |
| Rwork | 0.236 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.540 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Number of reflections | 105387 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 20% PEG 3350, 0.1M Bis Tris Propane pH 8.5, 0.23M sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
