4R7L

Structure of Human Leukotriene A4 Hydrolase in complex with inhibitor H1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Detector technology	CCD
Collection date	2014-04-11
Spacegroup name	P 21 21 21
Unit cell lengths	77.916, 87.134, 99.285
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.190 - 1.660
R-factor	0.15417
Rwork	0.153
R-free	0.18546
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	2.086
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.190	30.190
High resolution limit [Å]	1.660	1.660
Number of reflections	76480
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.8	277	15% PEG 8000, 0.1mM Na-acetate, 0.1mM Imidazole, 5mM YbCL3, pH 6.8, VAPOR DIFFUSION, temperature 277K