4R7I

Crystal structure of FMS kinase domain with a small molecular inhibitor, GLEEVEC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.18076
Spacegroup name	P 43 21 2
Unit cell lengths	63.130, 63.130, 183.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.640 - 2.750
R-factor	0.2024
Rwork	0.201
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hw7
RMSD bond length	0.003
RMSD bond angle	0.746
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	52.000
High resolution limit [Å]	2.300
Number of reflections	17142

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	20% PEG8K, 0.2M MGCL AND 0.1M TRIS, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K