4R6C
X-ray diffraction in temporally and spatially resolved biomolecular science: the X-ray crystal structure of hen egg white lysozyme cocrystallized with Ta6Br12 and then a crystal soaked in K2PtBr6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.070, 79.070, 37.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.360 - 1.700 |
R-factor | 0.1826 |
Rwork | 0.180 |
R-free | 0.23046 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.229 |
Data reduction software | SAINT |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.020 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.049 |
Number of reflections | 24843 |
Completeness [%] | 100.0 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch | 4.5 | 293 | NaCl; Ta6Br12 2mM. A crystal was soaked in K2PtBr6 10mM., pH 4.5, Batch, temperature 293K |