4R60
Crystal Structure of Xaa-Pro dipeptidase from Xanthomonas campestris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-06 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97947 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.324, 105.515, 111.352 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.462 - 1.830 |
R-factor | 0.1806 |
Rwork | 0.179 |
R-free | 0.20610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fkc |
RMSD bond length | 0.007 |
RMSD bond angle | 1.046 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.074 | 0.631 |
Number of reflections | 88109 | |
<I/σ(I)> | 18.1 | 2.1 |
Redundancy | 7 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch under-oil | 5.5 | 294 | 40mM KH2PO4, 15% glycerol, 12% PEG8000, pH 5.5, Microbatch under-oil, temperature 294K |