4R5S
Crystal structure of EGFR 696-1022 L858R in complex with FIIN-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97913 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 147.920, 147.920, 147.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.775 - 3.001 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.425 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.230 |
| High resolution limit [Å] | 3.000 | 5.130 | 3.000 |
| Number of reflections | 267837 | ||
| Completeness [%] | 99.9 | 99.9 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 40% PEG 400, 150mM NaCl, 0.1M HEPES (pH 8.0), 5mM TCEP, 0.1M NDSB-211, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
