4R4V

Crystal structure of the VS ribozyme - G638A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2011-11-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.07812
Spacegroup name	P 43 21 2
Unit cell lengths	102.229, 102.229, 215.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.072 - 3.070
R-factor	0.1802
Rwork	0.179
R-free	0.20400
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.827
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.130
High resolution limit [Å]	3.070	3.070
Number of reflections	21788
Completeness [%]	99.6	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M bis-Tris pH 6.5 2.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K