4R4M
Crystal structure of C42L cGMP dependent protein kinase I alpha (PKGI alpha) leucine zipper
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-10-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115869 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 95.926, 95.926, 63.805 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.921 - 1.922 |
| R-factor | 0.2246 |
| Rwork | 0.220 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WT PKGI alpha |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.714 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 1.922 |
| Number of reflections | 13609 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 40% PEG 400, 200 mM Lithium Sulfate, 0.1 M Tris base/ Hydrochloric acid, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
