4R4E
Structure of GlnR-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790, 0.97999, 0.9250 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 76.350, 103.300, 137.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.500 - 2.570 |
| R-factor | 0.2543 |
| Rwork | 0.254 |
| R-free | 0.27460 |
| Structure solution method | MAD |
| Starting model (for MR) | Same crystal form but selenomet substituted. This starting model was used for final refinement with the 2.52 A native data |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 82.500 |
| High resolution limit [Å] | 2.520 |
| Number of reflections | 17350 |
| <I/σ(I)> | 18.4 |
| Completeness [%] | 98.0 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 40% PEG 400, 0.1 M CAPS, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
