4R3R

Crystal structures of EGFR in complex with Mig6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97920
Spacegroup name	I 2 3
Unit cell lengths	144.689, 144.689, 144.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.755 - 3.250
R-factor	0.2009
Rwork	0.196
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.947
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	45.750	3.500
High resolution limit [Å]	3.260	5.550	3.250
Number of reflections	7110
Completeness [%]	87.8	82	92
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	40% PEG400, 0.15M SODIUM CHLORIDE, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K