4R3P

Crystal structures of EGFR in complex with Mig6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97920
Spacegroup name	I 2 3
Unit cell lengths	143.828, 143.828, 143.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.482 - 2.905
R-factor	0.211
Rwork	0.208
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.884
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.482	45.488	3.037
High resolution limit [Å]	2.900	4.609	2.905
Number of reflections	11009
Completeness [%]	99.2	97	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	40% PEG400, 0.15M Sodium chloride, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K