4R3I
The crystal structure of an RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-21 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.728, 39.748, 56.238 |
| Unit cell angles | 90.00, 103.56, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.2064 |
| Rwork | 0.203 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | a different crystal form of same protein |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.371 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.17) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.730 | 34.730 | 1.840 |
| High resolution limit [Å] | 1.800 | 8.990 | 1.800 |
| Rmerge | 0.112 | 0.041 | 0.587 |
| Rpim | 0.027 | 0.369 | |
| Total number of observations | 366 | 2551 | |
| Number of reflections | 14029 | ||
| <I/σ(I)> | 8.5 | 23.6 | 2.3 |
| Completeness [%] | 97.1 | 92 | 90.7 |
| Redundancy | 3.6 | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 25% PEG3350, 0.2 M amonium sulfate, 0.1 M bis-tris, pH 6.5, vapor diffusion, temperature 291K |
