4R3A

Erythrobacter litoralis EL346 blue-light activated histidine kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-15
Detector	ADSC QUANTUM 315r
Spacegroup name	H 3 2
Unit cell lengths	98.882, 98.882, 422.255
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.600 - 2.920
R-factor	0.294
Rwork	0.290
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r38 4r39
RMSD bond length	0.006
RMSD bond angle	1.050
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.920	2.950
Rmerge	0.089
Number of reflections	17699
Completeness [%]	99.8	100
Redundancy	33.6	27.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	293	26% PEG 3350, 0.2 M ammonium sulfate, 0.1 M BisTris, 0.1 mM reduced L-glutathione, 0.1 mM oxidized L-glutathione, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K