4R38

LOV domain from Erythrobacter litoralis EL346 blue-light activated histidine kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97901
Spacegroup name	P 21 21 21
Unit cell lengths	41.081, 89.383, 122.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.117 - 1.600
R-factor	0.201
Rwork	0.200
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pr5
RMSD bond length	0.010
RMSD bond angle	1.289
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.057	0.042	0.624
Number of reflections	58404
Completeness [%]	95.7	98.4	64.4
Redundancy	5	5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	15% PEG 4000, 0.1 M HEPES, 0.1 M magnesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K