4R30
Structure of human laforin dual specificity phosphatase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07 |
| Spacegroup name | I 41 |
| Unit cell lengths | 123.974, 123.974, 160.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.875 - 2.300 |
| R-factor | 0.1894 |
| Rwork | 0.188 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rgo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.159 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.600 | |
| Number of reflections | 52012 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 97.1 | 88.3 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.10 M lithium sulfate, 0.05 M sodium cacodylate trihydrate, 0.1 M NaCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
