4R1J

Crystal structure of Arc1p-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2014-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97871
Spacegroup name	P 21 21 21
Unit cell lengths	42.658, 49.961, 82.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.796 - 1.400
R-factor	0.178
Rwork	0.177
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fl0
RMSD bond length	0.006
RMSD bond angle	1.148
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.800	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.071	0.510
Number of reflections	35696
<I/σ(I)>	13.3	3
Completeness [%]	99.8	99.1
Redundancy	6.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1M MES, pH 6.0, 10 % v/v glycerol, 30 % w/v PEG 600 and 5 % w/v PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 293K