4R11
A conserved phosphorylation switch controls the interaction between cadherin and beta-catenin in vitro and in vivo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.057, 157.716, 84.824 |
| Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
| Resolution | 44.686 - 2.789 |
| R-factor | 0.2029 |
| Rwork | 0.199 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.740 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.789 | 6.030 | 2.789 |
| Rmerge | 0.073 | 0.032 | 0.655 |
| Number of reflections | 54788 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 99.3 | 99.3 | 95.9 |
| Redundancy | 3.6 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 17% PEG 3350, 0.1M Bis-Tris-Propane, 0.2M sodium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
