4R0Z
A conserved phosphorylation switch controls the interaction between cadherin and beta-catenin in vitro and in vivo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.225, 38.972, 101.128 |
| Unit cell angles | 90.00, 116.67, 90.00 |
Refinement procedure
| Resolution | 33.674 - 2.005 |
| R-factor | 0.1987 |
| Rwork | 0.196 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1I7W |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.747 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.038 | 0.025 | 0.222 |
| Number of reflections | 36385 | ||
| <I/σ(I)> | 17.1 | ||
| Completeness [%] | 93.0 | 97.3 | 81.2 |
| Redundancy | 4.5 | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.3M sodium formate, 50mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
