4R0D

Crystal structure of a eukaryotic group II intron lariat

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1052, 1.3854, 1.3858
Spacegroup name	C 2 2 21
Unit cell lengths	163.639, 255.361, 136.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.820 - 3.676
R-factor	0.2411
Rwork	0.239
R-free	0.27390
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.483
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.820	3.740
High resolution limit [Å]	3.676	3.680
Number of reflections	31107
<I/σ(I)>	6.4	0.6
Completeness [%]	99.9	99.9
Redundancy	6.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	303	0.4 mM spermine, 21% 2-Methyl-2,4-pentanediol, 175 mM magnesium acetate tetrahydrate, and 90 mM MES monohydrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 303K