4R0C
Crystal structure of the Alcanivorax borkumensis YdaH transporter reveals an unusual topology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-26 |
| Detector | ACSD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.145, 200.984, 101.493 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 48.434 - 2.963 |
| R-factor | 0.2086 |
| Rwork | 0.206 |
| R-free | 0.25020 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.351 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.090 |
| High resolution limit [Å] | 2.960 | 5.920 | 2.960 |
| Rmerge | 0.081 | 0.040 | 0.543 |
| Number of reflections | 74781 | ||
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 96.7 | 97 | 92.1 |
| Redundancy | 1.9 | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 30% PEG400, 0.05M Mg(Ac)2, 0.1M NaAc(5.0), and 3% glycerol, pH 7.5, vapor diffusion, temperature 298K |
