4QYZ
Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.2131 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 223.869, 223.869, 290.651 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.473 - 3.030 |
R-factor | 0.2257 |
Rwork | 0.224 |
R-free | 0.26720 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.524 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.473 | 3.050 |
High resolution limit [Å] | 3.030 | 3.030 |
Number of reflections | 163355 | |
Completeness [%] | 99.8 | 96.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 298 | 0.1 M sodium cacodylate, 0.1 M calcium acetate, 10% PEG 8,000, pH 5.0, VAPOR DIFFUSION, temperature 298K |