Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-07-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.78 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.507, 51.913, 82.705 |
| Unit cell angles | 89.07, 88.98, 66.83 |
Refinement procedure
| Resolution | 82.690 - 1.050 |
| R-factor | 0.11074 |
| Rwork | 0.110 |
| R-free | 0.13212 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2or3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.685 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.075 | 0.565 |
| Number of reflections | 309118 | |
| <I/σ(I)> | 21.6 | 2.1 |
| Completeness [%] | 97.3 | 90.2 |
| Redundancy | 5.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 26-32% PEG4000, 100 MM TRIS-HCL, 200 MM MAGNESIUM CHLORIDE, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
