4QY0
Structure of H10 from human-infecting H10N8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.026, 117.991, 123.766 |
| Unit cell angles | 98.29, 93.37, 96.56 |
Refinement procedure
| Resolution | 39.470 - 2.470 |
| R-factor | 0.2178 |
| Rwork | 0.215 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bsg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.891 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.470 | 2.500 |
| Number of reflections | 137551 | |
| Completeness [%] | 95.0 | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 15%(w/v) PEG 2000 MME, 0.1M potassium chloride, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
