4QY0
Structure of H10 from human-infecting H10N8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 74.026, 117.991, 123.766 |
Unit cell angles | 98.29, 93.37, 96.56 |
Refinement procedure
Resolution | 39.470 - 2.470 |
R-factor | 0.2178 |
Rwork | 0.215 |
R-free | 0.26350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bsg |
RMSD bond length | 0.005 |
RMSD bond angle | 0.891 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.470 | 2.500 |
Number of reflections | 137551 | |
Completeness [%] | 95.0 | 92.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 15%(w/v) PEG 2000 MME, 0.1M potassium chloride, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |