4QXP
Crystal structure of hSTING(G230I) in complex with DMXAA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2014-02-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.550 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.570, 77.919, 79.634 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.510 |
| R-factor | 0.1995 |
| Rwork | 0.198 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.262 |
| Data reduction software | RAPD |
| Data scaling software | RAPD |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 78.660 |
| High resolution limit [Å] | 2.510 |
| Number of reflections | 14320 |
| Completeness [%] | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 293 | 0.2 M Li2SO4, 20% PEG 3350, 0.015 mM CYMAL-7, 0.1 M Tris, pH 8.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
