4QXE
Crystal structure of LGR4 fused with hagfish VLR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-04-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | I 21 3 |
Unit cell lengths | 173.759, 173.759, 173.759 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.21003 |
Rwork | 0.209 |
R-free | 0.23733 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v44 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.123 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.290 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Rmerge | 0.116 | ||
Number of reflections | 44070 | ||
<I/σ(I)> | 4.5 | ||
Completeness [%] | 100.0 | 10.29 | 69.61 |
Redundancy | 28.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M HEPES pH 7.5, 2.0M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |