4QXB
crystal structure of histone demethylase KDM2A-H3K36ME3 with NOG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.604, 86.724, 171.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.550 - 1.600 |
| R-factor | 0.17049 |
| Rwork | 0.169 |
| R-free | 0.20701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.550 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.082 | 0.649 |
| Number of reflections | 107335 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.15 M CITRATE NA 20% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
