4QXA

Crystal structure of the Rab9A-RUTBC2 RBD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	52.776, 59.944, 127.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.725 - 2.300
R-factor	0.1831
Rwork	0.181
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yzl
RMSD bond length	0.009
RMSD bond angle	1.216
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.079	0.044	0.354
Number of reflections	18655
<I/σ(I)>	12.9
Completeness [%]	98.7	92.8	100
Redundancy	5.7	5.2	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	0.2M ammonium acetate, 0.1M sodium acetate, 20% (w/v) PEG 4000, pH 5.0, vapor diffusion, hanging drop, temperature 289K