4QX8
Crystal structure of histone demethylase kdm2a-h3k36me3 complex with alpha-kg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.797, 84.615, 170.077 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.040 - 1.650 |
| R-factor | 0.19007 |
| Rwork | 0.188 |
| R-free | 0.22668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.700 |
| Rmerge | 0.062 | |
| Number of reflections | 88250 | |
| <I/σ(I)> | 0.612 | |
| Completeness [%] | 96.6 | 95.5 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M Na Citrate, 18-20% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
