4QX6
CRYSTAL STRUCTURE OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM STREPTOCOCCUS AGALACTIAE NEM316 at 2.46 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.771, 107.855, 90.931 |
| Unit cell angles | 90.00, 106.57, 90.00 |
Refinement procedure
| Resolution | 64.960 - 2.460 |
| R-factor | 0.2056 |
| Rwork | 0.204 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3K73_Q |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.159 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.860 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.091 | 1.015 |
| Number of reflections | 45263 | |
| <I/σ(I)> | 13.8 | 1.3 |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 25% PEG4000, 0.1M Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
