4QWP
co-crystal structure of chitosanase OU01 with substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-04-22 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.235, 40.885, 105.313 |
| Unit cell angles | 90.00, 106.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4olt |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.940 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.058 |
| Number of reflections | 53026 |
| Completeness [%] | 97.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 290 | 0.05 M KH2PO4, 23% PEG 8000 using 0.7 M NaCl as the reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
