4QVZ
FMRP N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 89.538, 89.538, 118.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.560 - 3.195 |
| R-factor | 0.257 |
| Rwork | 0.254 |
| R-free | 0.30390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qw2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.742 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.560 | 29.561 | 3.657 |
| High resolution limit [Å] | 3.195 | 4.605 | 3.195 |
| Rmerge | 0.153 | 0.904 | |
| Number of reflections | 8207 | ||
| <I/σ(I)> | 13.25 | 2.529 | |
| Completeness [%] | 98.3 | 97 | 97 |
| Redundancy | 9.5 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 27% w/v PEG3350, 0.2 M ammonium sulfate, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
