4QVK
Apo-crystal structure of Podospora anserina methyltransferase PaMTH1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97626, 0.98401, 0.98004, 0.97989 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.384, 79.719, 84.393 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.267 - 1.970 |
R-factor | 0.1752 |
Rwork | 0.174 |
R-free | 0.20640 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.264 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 84.390 |
High resolution limit [Å] | 1.970 |
Rmerge | 0.067 |
Number of reflections | 35566 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 20% PEG 3350, 0.1M Bis-Tris, 0.2M NaCl, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |