4QVD
E.coli Hfq in complex with RNA Ads
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.280, 68.270, 111.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.130 - 1.972 |
| R-factor | 0.18699 |
| Rwork | 0.185 |
| R-free | 0.22923 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.256 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.190 | 2.080 |
| High resolution limit [Å] | 1.972 | 1.972 |
| Rmerge | 0.626 | |
| Number of reflections | 29189 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 92.0 | 91.2 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 281 | 16% MPEG5000, 0.1M Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
