4QU2

The structure of JMJD7 with alpha-KG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-28
Detector	NOIR-1
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	53.535, 59.516, 199.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.707 - 2.700
R-factor	0.2131
Rwork	0.208
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.550	2.550
Number of reflections	21313
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1 M MES monohydrate, pH 6.0, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K