4QTJ

Complex of WOPR domain of Wor1 in Candida albicans with the 13bp dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 32 2 1
Unit cell lengths	46.628, 46.628, 215.499
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.490 - 2.100
R-factor	0.20803
Rwork	0.206
R-free	0.24834
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.819
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.057	0.040	0.446
Number of reflections	16046
<I/σ(I)>	18.9
Completeness [%]	95.2	86.6	94
Redundancy	7.6	7.5	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	289	0.10M Bis-Tris, 0.2M ammonnuim sulfate, 20% PEG 3350, pH 5.7, temperature 289K, VAPOR DIFFUSION, HANGING DROP