4QT5
Crystal Structure of 3BD10: A Monoclonal Antibody against the TSH Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-11-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.979, 136.979, 149.071 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.860 - 2.500 |
| R-factor | 0.23766 |
| Rwork | 0.235 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k23 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.860 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.626 |
| Number of reflections | 32117 | |
| <I/σ(I)> | 7.6 | 1.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.4 | 291 | 20mM Tris.HCl, 11% PEG4K, pH 7.4, EVAPORATION, temperature 291K |
