4QSL
Crystal Structure of Listeria Monocytogenes Pyruvate Carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 91.360, 132.615, 257.544 |
| Unit cell angles | 86.65, 79.84, 70.07 |
Refinement procedure
| Resolution | 47.160 - 3.280 |
| R-factor | 0.23989 |
| Rwork | 0.238 |
| R-free | 0.28437 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bg5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.403 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.056 | 0.435 |
| Number of reflections | 151836 | |
| <I/σ(I)> | 11.3 | 1.6 |
| Completeness [%] | 89.4 | 72.2 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 16% PEG 3350, 0.1M BIS-TRIS pH 6.5, 1% Tacsimate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
